Size:96 Wells
$379.00
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SKU
OKEH00179
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MMP3 ELISA Kit (Human) : 96 Wells (OKEH00179)

Catalog#: OKEH00179
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species ReactivityHuman
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~ 3 Hours
Kit PrincipleAviva Systems Biology MMP3 ELISA Kit (Human) (OKEH00179) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP3 captured in well.
Kit Range78 - 5000pg/mL
Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Kit ReproducibilityMean Intra-assay CV%: < 3.8% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
Kit Component
ComponentAmount
MMP3 Microplate96 Wells (12 x 8 Well strips)
MMP3 Lyophilized Standard2
100X Biotinylated MMP3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity33 pg/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityNatural and recombinant Human Stromelysin-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMMP3
Official Gene Full Namematrix metallopeptidase 3
Alias SymbolsCHDS6, Matrix metalloproteinase-3, MGC126102, MGC126103, MGC126104, MMP-3, SL-1, STMY, STMY1, STR1, Stromelysin-1, Transin-1
NCBI Gene Id4314
Protein NameStromelysin-1
Description of TargetCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Swissprot IdP08254
Protein Accession #NP_002413
Nucleotide Accession #NM_002422
Protein Size (# AA)477
Molecular Weight54 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express MMP3.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express MMP3.
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