Search Antibody, Protein, and ELISA Kit Solutions

MMP3 ELISA Kit (Human) : 96 Wells (OKEH00179)

96 Wells
$449.00
In Stock
Request Bulk Order Quote

Predicted Species Reactivity:
Human
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
MMP3
Official Gene Full Name:
matrix metallopeptidase 3
Alias Symbols:
CHDS6, Matrix metalloproteinase-3, MGC126102, MGC126103, MGC126104, MMP-3, SL-1, STMY, STMY1, STR1, Stromelysin-1, Transin-1
NCBI Gene Id:
4314
Protein Name:
Stromelysin-1
Description of Target:
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Swissprot Id:
P08254
Protein Accession #:
NP_002413
Nucleotide Accession #:
NM_002422
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
33 pg/mL
Kit Range:
78 - 5000pg/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 3.8% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology MMP3 ELISA Kit (Human) (OKEH00179) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP3 captured in well.
Kit Component:
ComponentAmount
MMP3 Microplate96 Wells (12 x 8 Well strips)
MMP3 Lyophilized Standard2
100X Biotinylated MMP3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MMP3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MMP3.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Human Stromelysin-1
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Molecular Weight:
54 kDa
Protein Size (# AA):
477

Product Reviews

Protocol:
Tips Information:

Tell us what you think about this item!

Write A Review
    Please, wait...