Catalog No: OKEH00179
Size:96T
Price: $425.00
SKU
OKEH00179
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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MMP3 ELISA Kit (Human) : 96 Wells (OKEH00179)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH00179
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MMP3 ELISA Kit (Human) (OKEH00179) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP3 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.8% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
MMP3 Microplate96 Wells (12 x 8 Well strips)
MMP3 Lyophilized Standard2
100X Biotinylated MMP3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity33 pg/mL
SpecificityNatural and recombinant Human Stromelysin-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMMP3
Gene Full Namematrix metallopeptidase 3
Alias SymbolsCHDS6;matrix metalloproteinase 3 (stromelysin 1, progelatinase);Matrix metalloproteinase-3;MMP-3;proteoglycanase;SL-1;STMY;STMY1;STR1;stromelysin-1;transin-1.
NCBI Gene Id4314
Protein NameP08254
Description of TargetStromelysin-1
Uniprot IDhttps://www.uniprot.org/uniprot/P08254
Protein Accession #NP_002413
Nucleotide Accession #NM_002422
Protein Size (# AA)477
Molecular Weight54 kDa
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