Mmp23 ELISA Kit (Rat) (OKEH04775)

Catalog No: OKEH04775

Size:96T

Rat Mmp23 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Mmp23 ELISA Kit (Rat) (OKEH04775) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mmp23 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mmp23 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mmp23 captured in well.
• ELISA Kit Range: 1.56-100ng/ml
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Mmp23 Microplate: 96 Wells (12 x 8 Well Strips)
  • Mmp23 Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Mmp23 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.782 ng/mL
• Specificity: Natural and recombinant Rat Matrix metalloproteinase-23
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Mmp23
• Gene Full Name: matrix metallopeptidase 23
• Alias Symbols: Matrix metalloproteinase 23;matrix metalloproteinase-23;metalloprotease in the female reproductive tract;metaloprotease in the female reproductive tract;MIFR;MMP-23.
• NCBI Gene Id: 94339
• Protein Name: O88272
• Description of Target: Matrix metalloproteinase-23
• Uniprot ID: https://www.uniprot.org/uniprot/O88272
• Protein Accession #: NP_446058.1
• Nucleotide Accession #: NM_053606.2
• Protein Size (# AA): 391
• Molecular Weight: 45 kDa