Catalog No: OKEH08771
Size:96T
Price: $725.00
SKU
OKEH08771
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology MMP21 ELISA Kit (Human) (OKEH08771) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP21 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP21 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP21 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 108% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=5.1% (n = 20)
Mean Inter-assay CV%: <=11.1% (n = 20)
ELISA Kit Component
ComponentAmount
MMP21 Microplate96 Wells (12 x 8 Well strips)
MMP21 Lyophilized Standard2
100X Biotinylated MMP21 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Plays a specialized role in the generation of left-right asymmetry during embryogenesis. May act as a negative regulator of the NOTCH-signaling pathway (PubMed:26429889, PubMed:26437028). Cleaves alpha-1-antitrypsin (PubMed:12617721).
::Subcellular Location: Secreted
::Unigene: Hs.314141
SMR: Q8N119
String: 9606.ENSP00000357798
::KEGG: hsa:118856
MIM: 608416
Pfam: PF00045
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.083ng/mL
SpecificityNatural and recombinant Human Matrix metalloproteinase-21
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMMP21
Gene Full Namematrix metallopeptidase 21
Alias SymbolsHTX7;matrix metalloproteinase 21;matrix metalloproteinase-21;MMP-21.
NCBI Gene Id118856
Protein NameQ8N119
Description of TargetMatrix metalloproteinase-21
Uniprot IDhttps://www.uniprot.org/uniprot/Q8N119
Protein Accession #NP_671724.1
Nucleotide Accession #NM_147191.1
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