MMP2 ELISA Kit (Chicken) (OKEH07290)

Catalog No: OKEH07290

Size:96T

Chicken MMP2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology MMP2 ELISA Kit (Chicken) (OKEH07290) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP2 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.8% (n = 20)
  Mean Inter-assay CV%: <=8.9% (n = 20)
• ELISA Kit Component:
  • MMP2 Microplate: 96 Wells (12 x 8 Well strips)
  • MMP2 Lyophilized Standard: 2
  • 100X Biotinylated MMP2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
  
  Subcellular Location: Secreted Extracellular space Extracellular matrix
  Unigene: Gga.3199
  SMR: Q90611
  String: 9031.ENSGALP00000005656
  KEGG: gga:386583
  Pfam: PF00040
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.15ng/mL
• Specificity: Natural and recombinant Chicken 72 kDa type IV collagenase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MMP2
• Gene Full Name: matrix metallopeptidase 2
• Alias Symbols: 72 kDa gelatinase;72 kDa type IV collagenase;gelatinase A;matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase);matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase);matrix metalloproteinase-2;MMP-2.
• NCBI Gene Id: 386583
• Protein Name: Q90611
• Description of Target: 72 kDa type IV collagenase
• Uniprot ID: https://www.uniprot.org/uniprot/Q90611
• Protein Accession #: NP_989751.1
• Nucleotide Accession #: NM_204420.2