MMP1A Chemi-Luminescent ELISA Kit (Mouse) (OKCD03759)

Catalog No: OKCD03759

Size:96WELLS

Mouse MMP1A ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	85-102%	79-98%	83-97%	86-101%
  EDTA Plasma (n=5)	87-98%	83-99%	99-105%	91-102%
  Heparin Plasma (n=5)	80-103%	98-105%	87-101%	86-94%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 1 (MMP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 1 (MMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 1 (MMP1) level in the sample or standard.
• ELISA Kit Range: 27.4-20,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	96-104	101
  EDTA Plasma (n=5)	90-104	97
  Heparin Plasma (n=5)	84-98	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 1 (MMP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 1 (MMP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Mmp1a Microplate: 96 Wells (12 x 8 Well strips)
  • Mmp1a Lyophilized Standard: 2
  • 100X Mmp1a Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.312 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Mmp1a
• Gene Full Name: matrix metallopeptidase 1a (interstitial collagenase)
• Alias Symbols: collagenase-like A;interstitial collagenase A;matrix metalloproteinase 1a (interstitial collagenase);Matrix metalloproteinase-1a;Mco;Mcola;Mcol-A;MMP-1a.
• NCBI Gene Id: 83995
• Protein Name: Interstitial collagenase A
• Description of Target: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (By similarity). Able to degrade synthetic peptides and type I and II fibrillar collagen.
• Uniprot ID: Q9EPL5
• Protein Accession #: NP_114395
• Nucleotide Accession #: NM_032006