MMP16 Polyclonal Antibody (OABB00202)

Catalog No: OABB00202 (Formerly GWB-BBP125)

Size:100UG

Lane 1: Hela Cell Lysate
Lane 2: Colo320 Cell Lysate

IHC(P): Human Rectal Cancer Tissue

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Hamster|Human|Rat
• Product Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Immunohistochemistry|Immunohistochemistry-Frozen|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: The matrix metalloproteinase 16(MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14 , MMP15 , and MMP17. Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms. MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle.
  Background: The matrix metalloproteinase 16(MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q211. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14, MMP15, and MMP172. Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms2. MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle2
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
  Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Human, Rat
• Reference: 1. Sato, H.; Tanaka, M.; Takino, T.; Inoue, M.; Seiki, M. : Assignment of the human genes for membrane-type-1, -2, and -3 matrix metalloproteinases (MMP14, MMP15, and MMP16) to 14q12.2,
  16q12.2-q21, and 8q21, respectively, by in situ hybridization. Genomics 39: 412-413, 1997.
  2. Takino, T.; Sato, H.; Shinagawa, A.; Seiki, M. : Identification of the second membrane-type matrix metalloproteinase (MT-MMP-2) gene from a human placenta cDNA library: MT-MMPs form a unique membrane-type subclass in the MMP family. J. Biol. Chem. 270: 23013-23020, 1995
  
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Matrix metalloproteinase-16(MMP16) detection. Tested with WB, IHC-P, IHC-F in Human;Rat.

Target Info

• Gene Symbol: MMP16
• Gene Full Name: matrix metallopeptidase 16
• Alias Symbols: C8orf57;matrix metallopeptidase 16 (membrane-inserted);matrix metalloproteinase-16;membrane-type matrix metalloproteinase 3;membrane-type-3 matrix metalloproteinase;MMP-16;MMP-X2;MT3MMP;MT3-MMP;MT-MMP 3;MT-MMP2;MTMMP3;MT-MMP3;Putative transmembrane protein C8orf57.
• NCBI Gene Id: 4325
• Protein Name: Matrix metalloproteinase-16
• Description of Target: Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
• Uniprot ID: P51512
• Molecular Weight: 69521 MW