MMP16 ELISA Kit (Human) (OKCD07474)

Catalog No: OKCD07474

Size:96WELLS

MMP16 ELISA Kit (Human) (OKCD07474) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 16 (MMP16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 16 (MMP16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 16 (MMP16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Matrix Metalloproteinase 16 (MMP16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 16 (MMP16) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 16 (MMP16) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • MMP16 Microplate: 96 Wells (12 x 8 Well strips)
  • MMP16 Lyophilized Standard: 2
  • 100X MMP16 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.058ng/mL

Target Info

• Gene Symbol: MMP16
• Gene Full Name: matrix metallopeptidase 16
• Alias Symbols: C8orf57;matrix metallopeptidase 16 (membrane-inserted);matrix metalloproteinase-16;membrane-type matrix metalloproteinase 3;membrane-type-3 matrix metalloproteinase;MMP-16;MMP-X2;MT3MMP;MT3-MMP;MT-MMP 3;MT-MMP2;MTMMP3;MT-MMP3;Putative transmembrane protein C8orf57.
• NCBI Gene Id: 4325
• Protein Name: Matrix metalloproteinase-16
• Description of Target: Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
• Uniprot ID: P51512
• Protein Accession #: NP_005932.2
• Nucleotide Accession #: NM_005941.4