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Catalog No: OKEH06063
Size:96T
Price: $525.00
SKU
OKEH06063
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Mmp14 ELISA Kit (Rat) (OKEH06063) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mmp14 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Mmp14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mmp14 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 87%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <4.8%, n = 20
Mean Inter-Assay Precision - <7.7%, n = 20
ELISA Kit Component
ComponentAmount
MMP14 Microplate96 Wells (12 x 8 Well strips)
MMP14 Lyophilized Standard2
100X Biotinylated MMP14 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.162 ng/mL
SpecificityNatural and recombinant Rat Matrix metalloproteinase-14
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMmp14
Gene Full Namematrix metallopeptidase 14
Alias Symbolsmatrix metallopeptidase 14 (membrane-inserted);matrix metalloproteinase 14 membrane-inserted;matrix metalloproteinase-14;membrane type 1-matrix metalloproteinase;membrane-type matrix metalloproteinase 1;Membrane-type-1 matrix metalloproteinase;MMP-14;Mt1-mmp;MT1MMP;MT-MMP 1;MTMMP1.
NCBI Gene Id81707
Protein NameQ10739
Description of TargetMatrix metalloproteinase-14
Uniprot IDhttps://www.uniprot.org/uniprot/Q10739
Protein Accession #NP_112318.1
Nucleotide Accession #NM_031056.1
Protein Size (# AA)582
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