Catalog No: OPED00094
Size:10UG
Price: $462.00
SKU
OPED00094
Availability: Domestic: within 1-2 week delivery | International: 1-2 week
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for MMP13 Recombinant Protein (Human) (OPED00094) (OPED00094) |
---|
Predicted Species Reactivity | Homo sapiens|Human |
---|---|
Product Format | Liquid. In 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20 uM ZnCl2, 0.5% Brij-35, and 30% glycerol. |
Host | E. Coli |
Additional Information | Produced in E. coli. Active Matrix Metalloproteinase-13 (MMP-13, collagenase-3) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-13 (Tyr104-Asn274, NM_002427) with a C-terminal purification tag. This represents a |
Reconstitution and Storage | Store at -80C |
Purification | Purified by multi-step chromatography. |
Purity | >95% (SDS-PAGE) |
Biological Activity | Preincubation of MMP-13 catalytic domain at 13nM with the broad-spectrum inhibitor GM6001 at 20nM for 1 hour completely inhibits enzymatic activity.
Specific Activity: ≥100 U/µg. One U=100 pmol/min at 37°C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100 µM) as substrate. |
Application Info | Useful tool to study enzyme kinetics, cleave target substrates, and screen for inhibitors. |
Reference | Enzymatic, physicochemical and biological properties of MMP-sensitive alginate hydrogels: K.B. Fonseca, et al.; Soft Matter 9, 3283 (2013), Development and validation of novel enzyme activity methods to assess inhibition of matrix metalloproteinases (MMPs) in human serum by antibodies against enzyme therapeutics: T.J. Edkins, et al.; J. Pharm. Biomed. Anal. 70, 408 (2012), Abstract; Directed evolution of protease beacons that enable sensitive detection of endogenous MT1-MMP activity in tumor cell lines: A. Jabaiah, et al.; Chem. Biol. 18, 392 (2011), Abstract; Full Text The in vitro resistance of IgG2 to proteolytic attack concurs with a comparative paucity of autoantibodies against peptide analogs of the IgG2 hinge: R.J. Brezski, et al.; Mabs 3, 558 (2011), Abstract; Full Text The effect of a hydroxamic acid-containing polymer on active matrix metalloproteinases: G.A. Skarja, et al.; Biomaterials 30, 1890 (2009), Abstract; |
Gene Symbol | MMP13 |
---|---|
Gene Full Name | matrix metallopeptidase 13 |
Alias Symbols | CLG3;collagenase 3;MANDP1;matrix metalloproteinase 13 (collagenase 3);Matrix metalloproteinase-13;MDST;MMP-13. |
NCBI Gene Id | 4322 |
Protein Name | Collagenase 3 |
Description of Target | Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion. |
Uniprot ID | P45452 |
Protein Accession # | NP_003875.3 |
Nucleotide Accession # | NM_003884.4 |
Protein Size (# AA) | Recombinant |
Molecular Weight | 20 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review
We found other products you might like!