MMP13 ELISA Kit (Bovine) (OKEH07288)

Catalog No: OKEH07288

Size:96T

Bovine MMP13 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology MMP13 ELISA Kit (Bovine) (OKEH07288) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP13 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP13 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP13 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.8% (n = 20)
  Mean Inter-assay CV%: <=9.5% (n = 20)
• ELISA Kit Component:
  • MMP13 Microplate: 96 Wells (12 x 8 Well strips)
  • MMP13 Lyophilized Standard: 2
  • 100X Biotinylated MMP13 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
  Subcellular Location: Secreted Extracellular space Extracellular matrix Secreted
  Unigene: Bt.39
  SMR: O77656
  String: 9913.ENSBTAP00000020045
  KEGG: bta:281914
  Pfam: PF00045
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.081ng/mL
• Specificity: Natural and recombinant Bovine Collagenase 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MMP13
• Gene Full Name: matrix metallopeptidase 13
• Alias Symbols: collagenase 3;matrix metalloproteinase 13 (collagenase 3);matrix metalloproteinase-13;MMP-13.
• NCBI Gene Id: 281914
• Protein Name: O77656
• Description of Target: Collagenase 3
• Uniprot ID: https://www.uniprot.org/uniprot/O77656
• Protein Accession #: NP_776814.1
• Nucleotide Accession #: NM_174389.2