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MMP12 ELISA Kit (Human) : 96 Wells (OKEH00426)

96 Wells
$469.00
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Predicted Species Reactivity:
Human
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology MMP12 ELISA Kit (Human) (OKEH00426) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human MMP12 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human MMP12 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human MMP12 captured in well.
Kit Range:
0.156 - 10ng/mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
Kit Reproducibility:
Mean Intra-assay CV%: < 4.4% (n = 20)
Mean Inter-assay CV%: < 6.5% (n = 20)
Kit Component:
ComponentAmount
Anti-MMP12 Microplate96 Wells (12 x 8 Well strips)
MMP12 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated MMP121 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage:
Store as indicated in product manual.
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
0.039 ng/mL
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Human Macrophage metalloelastase
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol:
MMP12
Official Gene Full Name:
matrix metallopeptidase 12
Alias Symbols:
hME, HME, Macrophage elastase, Macrophage metalloelastase, Matrix metalloproteinase-12, ME, MGC138506, MME, MMP-12
NCBI Gene Id:
4321
Protein Name:
Macrophage metalloelastase
Description of Target:
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Swissprot Id:
P39900
Protein Accession #:
NP_002417.2
Nucleotide Accession #:
NM_002426.5
Protein Size (# AA):
470
Molecular Weight:
54 kDa
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MMP12.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MMP12.

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