MMP11 Recombinant Protein (Human) (OPED00092)

Catalog No: OPED00092

Size:10UG

Coomassie-stained SDS-PAGE showing a timecourse of α1-antitrypsin (3ug) cleavage after incubation at 37°C with or without MMP-11 catalytic domain. Lanes are as follows: 1. Marker; 2. 1.5 μg α1-antitrypsin (0 hours); 3. 50 ng MMP-11, (0 hours); 4. 1 μg MMP-11, (0 hours); 5. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (0 hours); 6. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (3 hours); 7. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (8 hours); 8. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (24 hours); 9. 1.5 μg α1-antitrypsin incubated with 1 μg MMP-11 (0 hours); 10. 1.5 μg α1-antitrypsin incubated with 1 μg MMP-11 (24 hours); 11. 1.5 μg α1-antitrypsin (24 hours); 12. Marker.

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Liquid 50mM TRIS, 5mM CaCl₂, 300mM NaCl, 20uM ZnCl₂, 0.5% Brij-35 and 30% glycerol
• Host: E. Coli
• Reconstitution and Storage: Store at -80°C
• Purification: Purified by multi-step chromatography.
• Concentration: 1 mg/ml
• Purity: >95% (SDS-PAGE)
• Biological Activity: Due to its unusual substrate preferences [A(A/Q)(N/A)~(L/Y)(T/V/M/R)(R/K), or G(G/A)E~LR5], MMP-11 cleaves MMP peptide substrates such as Prod. No. BML-P125, BML-P126, and BML-P132 extremely slowly (several hours yield very little product). Therefore, the activity of each lot of MMP-11 is verified by digestion of macromolecules.
• Application Info: Useful to study enzyme kinetics, cleave target substrates, and screen for inhibitors.
• Reference: Cancer cells, adipocytes and matrix metalloproteinase 11: a vicious tumor progression cycle: E.R. Motrescu & M.C. Rio; Biol. Chem. 389, 1037 (2008), Abstract;

Target Info

• Gene Symbol: MMP11
• Gene Full Name: matrix metallopeptidase 11
• Alias Symbols: matrix metallopeptidase 11 (stromelysin 3);Matrix metalloproteinase-11;SL-3;ST3;STMY3;stromelysin III;stromelysin-3.
• NCBI Gene Id: 4320
• Protein Name: Stromelysin-3
• Description of Target: May play an important role in the progression of epithelial malignancies.
• Uniprot ID: P24347
• Protein Accession #: NP_003833.4
• Nucleotide Accession #: NM_003842.4
• Protein Size (# AA): Recombinant
• Molecular Weight: 19 kDa