MMP11 Recombinant Protein (Human) (OPED00092)

Catalog No: OPED00092

Size:10 ug

Coomassie-stained SDS-PAGE showing a timecourse of α1-antitrypsin (3ug) cleavage after incubation at 37°C with or without MMP-11 catalytic domain. Lanes are as follows: 1. Marker; 2. 1.5 μg α1-antitrypsin (0 hours); 3. 50 ng MMP-11, (0 hours); 4. 1 μg MMP-11, (0 hours); 5. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (0 hours); 6. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (3 hours); 7. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (8 hours); 8. 1.5 μg α1-antitrypsin incubated with 50 ng MMP-11 (24 hours); 9. 1.5 μg α1-antitrypsin incubated with 1 μg MMP-11 (0 hours); 10. 1.5 μg α1-antitrypsin incubated with 1 μg MMP-11 (24 hours); 11. 1.5 μg α1-antitrypsin (24 hours); 12. Marker.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. In 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20 uM ZnCl2, 0.5% Brij-35, and 30% glycerol.
• Host: E. Coli
• Additional Information: MMP-11 may be a link between obesity and cancer.
• Reconstitution and Storage: Store at -80C
• Purification: Purified by multi-step chromatography.
• Purity: >95% (SDS-PAGE)
• Application Info: Useful to study enzyme kinetics, cleave target substrates, and screen for inhibitors.
• Reference: Cancer cells, adipocytes and matrix metalloproteinase 11: a vicious tumor progression cycle: E.R. Motrescu & M.C. Rio; Biol. Chem. 389, 1037 (2008), Abstract;

Target Info

• Gene Symbol: MMP11
• Gene Full Name: matrix metallopeptidase 11
• Alias Symbols: ST3, SL-3, STMY3
• NCBI Gene Id: 4320
• Protein Name: Stromelysin-3
• Description of Target: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is activated intracellularly by furin within the constitutive secretory pathway. Also in contrast to other MMP's, this enzyme cleaves alpha 1-proteinase inhibitor but weakly degrades structural proteins of the extracellular matrix.
• Uniprot ID: P24347
• Protein Accession #: NP_003833.4
• Nucleotide Accession #: NM_003842.4
• Protein Size (# AA): 488
• Molecular Weight: 19 kDa