MMP11 ELISA Kit (Human) (OKCD06158)

Catalog No: OKCD06158

Size:96WELLS

MMP11 ELISA Kit (Human) (OKCD06158) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 11 (MMP11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 11 (MMP11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 11 (MMP11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Matrix Metalloproteinase 11 (MMP11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 11 (MMP11) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 11 (MMP11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • MMP11 Microplate: 96 Wells (12 x 8 Well strips)
  • MMP11 Lyophilized Standard: 2
  • 100X MMP11 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.27ng/mL

Target Info

• Gene Symbol: MMP11
• Gene Full Name: matrix metallopeptidase 11
• Alias Symbols: matrix metallopeptidase 11 (stromelysin 3);Matrix metalloproteinase-11;SL-3;ST3;STMY3;stromelysin III;stromelysin-3.
• NCBI Gene Id: 4320
• Protein Name: Stromelysin-3
• Description of Target: May play an important role in the progression of epithelial malignancies.
• Uniprot ID: P24347
• Protein Accession #: NP_005931.2
• Nucleotide Accession #: NM_005940.4