MMP10 Chemi-Luminescent ELISA Kit (Rat) (OKCD03972)

Catalog No: OKCD03972

Size:96WELLS

Rat MMP10 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	91-99%	89-96%	86-103%	94-102%
  EDTA Plasma (n=5)	87-94%	82-93%	95-102%	78-95%
  Heparin Plasma (n=5)	80-101%	84-98%	96-105%	97-104%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 10 (MMP10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 10 (MMP10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 10 (MMP10) level in the sample or standard.
• ELISA Kit Range: 6.86-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	88-96	93
  EDTA Plasma (n=5)	78-102	82
  Heparin Plasma (n=5)	83-98	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 10 (MMP10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 10 (MMP10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Mmp10 Microplate: 96 Wells (12 x 8 Well strips)
  • Mmp10 Lyophilized Standard: 2
  • 100X Mmp10 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Mmp10
• Gene Full Name: matrix metallopeptidase 10
• Alias Symbols: matrix metalloproteinase-10;SL-2;stromelysin-2;transformation-associated protein 34A;transin-2.
• NCBI Gene Id: 117061
• Protein Name: Stromelysin-2
• Description of Target: Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
• Uniprot ID: P07152
• Protein Accession #: NP_598198
• Nucleotide Accession #: NM_133514