MMP1 ELISA Kit (Sheep) (OKCD04239)

Catalog No: OKCD04239

Size:96 Wells

Sheep MMP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Sheep
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-88%	79-92%	92-103%	79-98%
  Heparin Plasma (n=5)	84-96%	91-105%	89-97%	90-98%

• ELISA Kit Principle: Aviva Systems Biology MMP1 ELISA Kit (Sheep) (OKCD04239) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP1 captured in the well.
• ELISA Kit Range: 0.312-20 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	85-105	89
  Heparin Plasma (n=5)	84-104	101

• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level MMP1. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level MMP1 using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • Mmp1 Microplate: 96 Wells (12 x 8 Well strips)
  • Mmp1 Lyophilized Standard: 2
  • 100X Mmp1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: 0.113 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MMP1
• Alias Symbols: TIMP-1
• NCBI Gene Id: 443331
• Protein Name: metalloproteinase inhibitor 1
• Uniprot ID: P50122
• Protein Accession #: NP_001009319.1
• Nucleotide Accession #: NM_001009319.2