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Catalog No: OKCA02238
Size:96 Wells
Price: $667.00
SKU
OKCA02238
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
Sample TypeDilutionMean (%)Range (%)
Serum (n=4)1:1008985-94
Serum (n=4)1:2009287-98
Serum (n=4)1:4009590-102
Serum (n=4)1:80010096-109
ELISA Kit PrincipleAviva Systems Biology MMP1 ELISA Kit (Rat) (OKCA02238) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MMP1 captured in well.
ELISA Kit Range62.5 pg/mL-4,000 pg/mL
ELISA Kit Recovery
Sample TypeMean (%)Range (%)
Serum (n=5)8480-89
EDTA plasma (n=4)9790-104
ELISA Kit ReproducibilityMean Intra-Assay Variation <10% CV (n=20)
Mean Inter-Assay Variation <12% CV (n=8)
ELISA Kit Component
ComponentAmount
Anti-MMP1 Microplate96 Wells (12 x 8 Well strips)
MMP1 Lyophilized Standard 2 x 4 ng
100X Biotinylated MMP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Culture Supernates, Tissue Homogenates, Cell Lysates
Sensitivity15.6 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMMP1
Alias SymbolsMMP-1
NCBI Gene Id100009110
Protein NameInterstitial collagenase
Description of TargetCleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X.
Uniprot IDP13943
Protein Accession #NP_001164610
Nucleotide Accession #NM_001171139
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