Catalog No: OKCA02238
Size:96 Wells
Price: $667.00
SKU
OKCA02238
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Rat | ||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology MMP1 ELISA Kit (Rat) (OKCA02238) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MMP1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 62.5 pg/mL-4,000 pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay Variation <10% CV (n=20) Mean Inter-Assay Variation <12% CV (n=8) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Cell Culture Supernates, Tissue Homogenates, Cell Lysates | ||||||||||||||||||||||
Sensitivity | 15.6 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | MMP1 |
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Alias Symbols | MMP-1 |
NCBI Gene Id | 100009110 |
Protein Name | Interstitial collagenase |
Description of Target | Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. |
Uniprot ID | P13943 |
Protein Accession # | NP_001164610 |
Nucleotide Accession # | NM_001171139 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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