Size:96 Wells
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MMP1 ELISA Kit (Human) : 96 Wells (OKEH00170)

Catalog#: OKEH00170
Domestic: within 1 week delivery International: 1 week
More Information
Predicted Species Reactivity Human
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology MMP1 ELISA Kit (Human) (OKEH00170) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP1 captured in well.
Kit Range 0.156 - 10ng/mL
Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Kit Reproducibility Mean Intra-assay CV%: < 3.9% (n = 20)
Mean Inter-assay CV%: < 7.6% (n = 20)
Kit Component
MMP1 Microplate96 Wells (12 x 8 Well strips)
MMP1 Lyophilized Standard2
100X Biotinylated MMP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity 0.039 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Human Interstitial collagenase
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol MMP1
Official Gene Full Name matrix metallopeptidase 1
Alias Symbols CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase-1, MMP-1
NCBI Gene Id 4312
Protein Name Interstitial collagenase
Description of Target Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
Swissprot Id P03956
Protein Accession # NP_001139410
Nucleotide Accession # NM_001145938
Protein Size (# AA) 469
Molecular Weight 54 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express MMP1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express MMP1.
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