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Catalog No: OKCD03761
Size:96WELLS
Price: $875.00
SKU
OKCD03761
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MMP1 Chemi-Luminescent ELISA Kit (Rabbit) (OKCD03761)

Datasheets/ManualsPrintable datasheet for MMP1 Chemi-Luminescent ELISA Kit (Rabbit) (OKCD03761)
Product Info
Predicted Species ReactivityOryctolagus cuniculus|Rabbit
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)81-88%88-96%88-96%92-105%
EDTA Plasma (n=5)80-101%79-103%89-103%81-90%
Heparin Plasma (n=5)89-96%95-104%88-95%93-101%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 1 (MMP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 1 (MMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 1 (MMP1) level in the sample or standard.
ELISA Kit Range2.74-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)91-10195
EDTA Plasma (n=5)84-9990
Heparin Plasma (n=5)87-9590
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 1 (MMP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 1 (MMP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Mmp1 Microplate96 Wells (12 x 8 Well strips)
Mmp1 Lyophilized Standard2
100X Mmp1 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity31.2 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMMP1
Gene Full Namematrix metallopeptidase 1
Alias Symbolscollagenase-1;interstitial collagenase;matrix metallopeptidase 1 (interstitial collagenase);matrix metalloproteinase-1;MMP-1.
NCBI Gene Id100009110
Protein NameInterstitial collagenase
Description of TargetCleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X.
Uniprot IDP13943
Protein Accession #NP_001164610
Nucleotide Accession #NM_001171139
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