Catalog No: OKBB00219 (Formerly GWB-ZZD109)
Size:96 Tests
Price: $583.00
SKU
OKBB00219
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's human MMP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-1 has been precoated onto 96-well plates. Standards(NS0, F20-N469) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human MMP-1 amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (pg/mL)93731275438125934565862
St. Dev.42.2159.5293.774.3207.4393
%CV4.505.105.405.906.006.70
ELISA Kit Component
ComponentAmount
Lyophilized recombinant human MMP-1standard10 ng/tube x 2
Anti-human MMP-1 Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti-human MMP-1 antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Additional InformationRange: 156pg/ml-10,000pg/ml
::Principle: Aviva’s human MMP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human MMP-1 specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-1 amount of sample captured in plate.
::Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Matrix metalloproteinase-1 (MMP-1) is also known as collagenase. Matrix metalloproteinases (MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1 (MMP-1) plays an important role in the degradation of collagen in inflammatory diseases.1 MMP1 (interstitial collagenase), MMP9 (gelatinase B) and MMP12 (macrophage elastase) are thought to be important in the development of emphysema.2 Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking.3 The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typecell culture supernates, serum and plasma (heparin)
Sensitivity<20 pg/ml
Predicted Homology Based on Immunogen SequenceNo detectable cross-reactivity with any other cytokine.
Reference1. Domeij, H.; Modeer, T.; Quezada, HC.; Yucel-Lindberg, T. Cell expression of MMP-1 and TIMP-1 in co-cultures of
human gingival fibroblasts and monocytes: the involvement of ICAM-1. Biochem. Biophys. Res. Commun. 2005 Dec
30;338(4):1825-33. Epub 2005 Nov 2.
2. Joos, L.; He, J.-Q.; Shepherdson, M. B.; Connett, J. E.; Anthonisen, N. R.; Pare, P. D.; Sandford, A. J. The role of
matrix metalloproteinase polymorphisms in the rate of decline in lung function. Hum. Molec. Genet. 11: 569-576,
2002.
3. Lahmann, C.; Bergemann, J.; Harrison, G.; Young, A. R. Matrix metalloproteinase-1 and skin ageing in smokers.
(Letter) Lancet 357: 935-936, 2001.
SpecificityNatural and recombinant human total MMP-1
ImmunogenExpression system for standard: NS0; Immunogen sequence: F20-N469
DescriptionFor quantitative detection of human MMP-1 in cell culture supernatants, serum and plasma(heparin).
Gene SymbolMMP1
Gene Full Namematrix metallopeptidase 1 (interstitial collagenase)
Alias Symbols27 kDa interstitial collagenase, CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metallopeptidase 1 (interstitial collagenase), Matrix Metalloproteinase 1, Matrix metalloproteinase-1, MMP 1, MMP-1, MMP1, MMP1_HUMAN
NCBI Gene Id4312
Protein NameInterstitial collagenase
Description of TargetMatrix metalloproteinase-1 (MMP-1) is also known as collagenase. Matrix metalloproteinases (MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1 (MMP-1) plays an important role in the degradation of collagen in inflammatory diseases.1 MMP1 (interstitial collagenase), MMP9 (gelatinase B) and MMP12 (macrophage elastase) are thought to be important in the development of emphysema.2 Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking.3 The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.
Uniprot IDP03956
Protein Accession #NP_002412.1
Nucleotide Accession #NM_002421.3
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