MME Antibody : APC (OATA00029)

Catalog No: OATA00029

Human peripheral blood granulocytes were stained with 5 uL (0.25 ug) APC Anti-Human CD10 (OATA00029) (solid line) or 0.25 ug APC Mouse IgG1 isotype control (dashed line).

Human peripheral blood granulocytes were stained with the manufacturers recommended amount of APC Anti-Human CD10 (SN5c) manufactured by Aviva Systems Biology (left panel) or eBioscience (right panel).

Product Info

• Tested Species Reactivity: Human
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: SN5c
• Isotype: Mouse IgG1, kappa
• Host: Mouse
• Conjugation: APC: Allophycocyanin
• Application: FC
  Excitation Laser: Red (633-640 nm)
  Emmision: 660 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD10
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
• Reference:

  Matsuzaki H, Haruta Y, Fukukawa T, Barcos MP and Seon BK. 1987. Cancer Res. 47(8): 2160-2166.

  Shipp MA, Stefano GB, Switzer SN, Griffin JD and Reinherz EL. 1991. Blood. 78(7): 1834-1841.

  Pinho S, Lacombe J, Hanoun M, Mizoguchi T, Bruns I, Kunisaki Y and Frenette PS. 2013. J Exp Med. 201(7): 1351-1367. (Flow cytometry)

  Mukhopadhyay C, Zhao X, Maroni D, Band V and Naramura M. 2013. PLoS One. 8(10): e75907. (Flow cytometry)

Target Info

• Gene Symbol: MME
• Gene Full Name: membrane metalloendopeptidase
• Alias Symbols: NEP, SFE, CD10, CALLA, CMT2T, SCA43
• NCBI Gene Id: 4311
• Protein Name: neprilysin
• Description of Target: The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.
• Uniprot ID: P08473
• Protein Accession #: NP_000893.2
• Nucleotide Accession #: NM_000902.3