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Catalog No: OKDD00392 (Formerly GWB-KBBNQ3)
Size:96 Wells
Price: $800.00
SKU
OKDD00392
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Datasheets/ManualsPrintable datasheet for MLH1 ELISA Kit (Human) (OKDD00392)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology MLH1 ELISA Kit (Human) (OKDD00392) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for MLH1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MLH1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MLH1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MLH1 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MLH1 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-MLH1 Microplate96 Wells (12 x 8 Well Strips)
MLH1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X MLH1 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell lysates
Sensitivity< 0.057 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of MLH1. No significant cross-reactivity or interference between MLH1 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolMLH1
Gene Full NamemutL homolog 1
Alias SymbolsCOCA2;DNA mismatch repair protein Mlh1;FCC2;hMLH1;HNPCC;HNPCC2;MMRCS1;mutL homolog 1, colon cancer, nonpolyposis type 2;MutL protein homolog 1.
NCBI Gene Id4292
Protein NameDNA mismatch repair protein Mlh1
Description of TargetHeterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
Uniprot IDP40692
Protein Accession #NP_000240.1
Nucleotide Accession #NM_000249.3
Protein Size (# AA)756
Molecular Weight85 kDa
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