Catalog No: OKCD00857 (Formerly GWB-KBBPP4)
Size:96 Wells
Price: $700.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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MKI67 ELISA Kit (Mouse) (OKCD00857)

100% of 100
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva Systems Biology Mki67 ELISA Kit (Mouse) (OKCD00857) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mki67 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mki67 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Mki67captured in well.
ELISA Kit Range0.312-20 ng/mL
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level Mki67 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Mki67 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
Anti-Mki67 Microplate96 Wells (12 x 8 Well Strips)
Mki67 Lyophilized Standard2 x 20 ng
100X Biotinylated Mki67 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.119 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ki-67 Protein (Ki67P). No significant cross-reactivity or interference between Ki-67 Protein (Ki67P) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMKI67
Gene Full Nameantigen identified by monoclonal antibody Ki 67
Alias SymbolsKi6, Ki-6, Ki67, Ki-67, D630048A14Rik
NCBI Gene Id17345
Protein Nameproliferation marker protein Ki-67
Swissprot IdE9PVX6
Protein Accession #NP_001074586
Nucleotide Accession #NM_001081117.2
Protein Size (# AA)3177
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express MKI67.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express MKI67.
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