Ki-67 ELISA kit (Mouse) (OKCD00857)

Catalog No: OKCD00857 (Formerly GWB-KBBPP4)

Size:96WELLS

Mouse Mki67 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: ELISA-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ki-67 Protein (Ki-67). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ki-67 Protein (Ki-67). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ki-67 Protein (Ki-67), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ki-67 Protein (Ki-67) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ki-67 Protein (Ki-67) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ki-67 Protein (Ki-67) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Mki67 Microplate: 96 Wells (12 x 8 Well Strips)
  • Mki67 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Mki67 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.119 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ki-67 Protein (Ki67P). No significant cross-reactivity or interference between Ki-67 Protein (Ki67P) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Mki67
• Gene Full Name: antigen identified by monoclonal antibody Ki 67
• Alias Symbols: antigen identified by monoclonal antibody Ki-67 homolog;antigen Ki67 homolog;antigen KI-67 homolog;D630048A14Rik;Ki6;Ki-6;Ki67;Ki-67;proliferation marker protein Ki-67.
• NCBI Gene Id: 17345
• Protein Name: Proliferation marker protein Ki-67
• Description of Target: Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
• Uniprot ID: E9PVX6
• Protein Accession #: NP_001074586
• Nucleotide Accession #: NM_001081117.2
• Protein Size (# AA): 3177