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MKI67 ELISA Kit (Mouse) (OKCD00857)

96 Wells
$629.00
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Application:
ELISA
Gene Symbol:
MKI67
Official Gene Full Name:
antigen identified by monoclonal antibody Ki 67
NCBI Gene Id:
17345
Alias Symbols:
MKI67, KIA, Ki-67, Antigen Identified By Monoclonal Antibody Ki-67
Protein Name:
proliferation marker protein Ki-67
Swissprot Id:
E9PVX6
Protein Accession #:
NP_001074586
Nucleotide Accession #:
NM_001081117.2
Predicted Species Reactivity:
Mouse
Sample Type:
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
< 0.119 ng/mL
Kit Range:
0.312-20 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level Mki67 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Mki67 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology Mki67 ELISA Kit (Mouse) (OKCD00857) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mki67 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mki67 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Mki67captured in well.
Kit Component:
ComponentAmount
Anti-Mki67 Microplate96 Wells (12 x 8 Well Strips)
Mki67 Lyophilized Standard2 x 20 ng
100X Biotinylated Mki67 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MKI67.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MKI67.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Ki-67 Protein (Ki67P). No significant cross-reactivity or interference between Ki-67 Protein (Ki67P) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
3177

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10/01/2018 15:17
  • Overall Experience:
  • Quality:
Cell lysates in ELISA

Anonymously submitted through Aviva's ELISA kit sampling program.

"ELISA kit was performed according to instructions"

 

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