- Description of Target:
Mix-n-Stain#8482; HRP antibody labeling kits contain everything you need to rapidly conjugate an antibody to horseradish peroxidase (HRP). Choose the kit size corresponding to the amount of antibody you wish to label. After labeling, the HRP conjugate is stable for one month when stored at 4oC.
Mix-n-Stain labeling can tolerate low levels of glycerol. A microcentrifuge ultrafiltration vial is provided in the kit to rapidly remove incompatible small molecule antibody stabilizers such as sodium azide, Tris, glycine, or excess glycerol before labeling (see Table 1). Labeling can be performed in the presence of up to four-fold excess of BSA or gelatin to IgG (by ug amount).
Aviva also offers Mix-n-Stain labeling kits for labeling antibodies with one of Aviva’s next-generation fluorescent CF#8482; dyes, biotin, or FITC.
- Printable datasheet/reference manual for Mix-n-Stain HRP Antibody Labeling Kit, 1x(25-50ug) labeling (OKBE00065) OKBE00065
- Reconstitution and Storage:
Stability: Stable for at least 3 months from date of receipt when stored as recommended.
|Component||Catalog# OKBE00065: 25-50 ug labeling|
|Modified HRP||Part # 92301A -(1) vial |
|Reaction buffer|| Part # 99994 -25 uL |
|Reaction enhancer||Part # 99995- (1) vial|
|Storage buffer||Part # 99996-150uL- 150 uL|
|Ultrafiltration vial (MWCO=10K)||1 each Part # 99956|
- Please visit www.avivasysbio.com to view our full selection of products including CF#8482; dye Mix-n-Stain antibody labeling kits, secondary antibodies, streptavidin, anti-biotin, and anti-tag antibodies. Aviva also offers a variety of apoptosis and cell viability assays for flow cytometry analysis, including mitochondrial membrane potential dyes, fluorescent Annexin V conjugates, and NucView™488 Caspase-3 Substrate for live cells.
Aviva products are high-quality reagents and materials intended for research purposes only. Some products are potentially hazardous chemicals - please read the Material Safety Data Sheet for additional information regarding handling potentially hazardous chemicals. Several of Aviva products and product applications are covered by U.S. and international patents and pending patents. Our products are not available for resale or other commercial uses without a specific agreement from Aviva Systems Biology Corporation.. We welcome inquiries about licensing the use of our dyes, trademarks or technologies. Please submit inquiries by e-mail to firstname.lastname@example.org.
- Mix-n-StainTM Antibody Compatibility and Labeling Protocol Selection Guide
|Sodium Azide||Perform ultrafiltration (Section A)|
|Glycerol||Up to 10%: Compatible, proceed to Section B|
Greater than 10%: Perform ultrafiltration (Section A)
|Tris||Perform ultrafiltration (Section A)|
|Glycine||Perform ultrafiltration (Section A)|
|BSA or gelatin||Up to 4X IgG (ug amount): Compatible, proceed to Section B|
More than 4X IgG (ug amount): Not compatible, purify IgG
|Ascites fluid||Not compatible, purify IgG|
|Serum||Not compatible; purify IgG|
|Hybridoma supernatant||Not compatible: purify IgG|
- Tips Information: