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Catalog No: OKEH04343
Size:96T
Price: $525.00
SKU
OKEH04343
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MITF ELISA Kit (Human) (OKEH04343) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MITF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for MITF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MITF captured in the well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.2% (n = 20)
Mean Inter-assay CV%: <= 9.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-MITF Microplate96 Wells (12 x 8 Well Strips)
MITF Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
MITF Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.35 ng/mL
SpecificityNatural and recombinant Human Microphthalmia-associated transcription factor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMITF
Gene Full Namemelanocyte inducing transcription factor
Alias SymbolsbHLHe32;class E basic helix-loop-helix protein 32;CMM8;COMMAD;melanogenesis associated transcription factor;MI;microphtalmia-associated transcription factor;microphthalmia-associated transcription factor;MITF-A;WS2;WS2A.
NCBI Gene Id4286
Protein NameO75030
Description of TargetMicrophthalmia-associated transcription factor
Uniprot IDhttps://www.uniprot.org/uniprot/O75030
Protein Accession #NP_000239.1
Nucleotide Accession #NM_000248.3
Protein Size (# AA)526
Molecular Weight59 KDa
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