Catalog No: OKAG00153 (Formerly GWB-SKR153)
Size:12x8WELLS
Price: $475.00
SKU
OKAG00153
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
Product Format1 x 96-Well Plate or 12 x 8-Well Strips
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric 450 nm
ELISA Kit Duration4.5 hours
ELISA Kit PrincipleThe Aviva Human MIP-1δELISA Kit contains the components necessary for quantitative determination of natural or recombinant hMIP-1δ concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator.The capture antibodies coated to the bottom of each well are specific for a particular epitope on theHuman MIP-1δ cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetricreaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
ELISA Kit Range16-1000 pg/ml
ELISA Kit Component
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (17 ng)
Reconstitution and Storage2°C to 8°C
Sample TypeCell lysates, sera and plasma
Sensitivity16 pg/mL
SpecificityThe Human MIP-1δ ELISA is capable of recognizing both recombinant and naturally produced Human MIP-1δ proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: HCC-1, IL-7R, MPIF-1, Neuritin
• Murine: C-10, MIP-1γ
• Rat: Jagged
Assay InfoQuantitative Colorimentric Sandwich ELISA
Gene SymbolCCL15
Gene Full NameC-C motif chemokine ligand 15
Alias SymbolsC-C motif chemokine 15;chemokine (C-C motif) ligand 15;chemokine CC-2;HCC-2;HMRP-2B;leukotactin 1;Leukotactin-1;LKN1;LKN-1;macrophage inflammatory protein 5;MIP-1 delta;MIP-1D;MIP-5;MRP-2B;NCC3;NCC-3;new CC chemokine 3;SCYA15;SCYL3;small inducible cytokine subfamily A (Cys-Cys), member 15;small-inducible cytokine A15;SY15.
NCBI Gene Id6359
Protein NameQ16663
Description of TargetC-C motif chemokine 15
Uniprot IDhttps://www.uniprot.org/uniprot/Q16663
Protein Accession #NP_116741.2
Nucleotide Accession #NM_032965.5
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