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Catalog No: OKCD02058 (Formerly GWB-KBBXQ8)
Size:96WELLS
Price: $750.00
SKU
OKCD02058
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Datasheets/ManualsPrintable datasheet for MINA ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYC Induced Nuclear Antigen (MINA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MYC Induced Nuclear Antigen (MINA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYC Induced Nuclear Antigen (MINA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MYC Induced Nuclear Antigen (MINA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MYC Induced Nuclear Antigen (MINA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MYC Induced Nuclear Antigen (MINA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MINA Microplate96 Wells (12 x 8 Well Strips)
MINA Lyophilized Standard2 x 10 ng
100X Biotinylated MINA Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.056 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of MYC Induced Nuclear Antigen (MINA). No significant cross-reactivity or interference between MYC Induced Nuclear Antigen (MINA) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMINA
Gene Full NameMYC induced nuclear antigen
Alias SymbolsROX, MDIG, MINA, NO52, JMJD10, MINA53
NCBI Gene Id84864
Protein NameBifunctional lysine-specific demethylase and histidyl-hydroxylase MINA
Description of TargetOxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression. Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
Uniprot IDQ8IUF8
Protein Accession #NP_001035998.1
Nucleotide Accession #NM_001042533.2
Protein Size (# AA)465
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