MIF Recombinant Protein (OPCD05271)

Catalog No: OPCD05271

MIF Recombinant Protein (OPCD05271) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Product Format: Lyophilized 20mM Tris, 150mM NaCl (pH 8.0) containing 0.01% SKL, 5% Trehalose
• Host: E.coli
• Conjugation: Unconjugated
• Application: Ctrl (+), SDS-PAGE, WB
• Additional Information: Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
  Residues: Phe4-Ser112
  Isoelectric Point: 6.7
  Subcellular Location: Secreted, Cytoplasm
• Reconstitution and Storage: Reconstitute in 20mM Tris, 150mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/ml. Do not vortex. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. Avoid repeated freeze/thaw cycles.
• Concentration: 700 ug/mL (prior to lyoph)
• Purity: > 97%
• Source: E.coli
• Tag: N-terminal His Tag

Target Info

• Gene Symbol: LOC103694877|Mif
• Gene Full Name: macrophage migration inhibitory factor
• Alias Symbols: glutathione-binding 13 kDa protein;L-dopachrome isomerase;L-dopachrome tautomerase;macrophage migration inhibitory factor;macrophage migration inhibitory factor (glycosylation-inhibiting factor);phenylpyruvate tautomerase.
• NCBI Gene Id: 103694877|81683
• Protein Name: Macrophage migration inhibitory factor
• Description of Target: Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (By similarity).
• Uniprot ID: P30904
• Protein Accession #: NP_112313.1
• Nucleotide Accession #: NM_031051.1
• Protein Size (# AA): Phe4~Ser112
• Molecular Weight: 13 kDa