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Catalog No: OKEH02830
Size:96T
Price: $525.00
SKU
OKEH02830
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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MICA ELISA Kit (Human) : 96 Wells (OKEH02830)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MICA ELISA Kit (Human) (OKEH02830) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MICA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MICA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MICA captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-MICA Microplate96 Wells (12 x 8 Well strips)
MICA Lyophilized Standard2
100X Biotinylated MICA Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity33 pg/mL
SpecificityNatural and recombinant Human MHC class I polypeptide-related sequence A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMICA
Gene Full NameMHC class I polypeptide-related sequence A
Alias SymbolsHLA class I antigen;major histocompatibility complex class I chain-related protein A;MHC class I chain-related protein A;MHC class I polypeptide-related sequence A;MHC class I related chain A;MHC class I related sequence A;MIC-A;PERB11.1;stress inducible class I homolog;truncated MHC class I polypeptide-related sequence A;truncated MICA.
NCBI Gene Id100507436
Protein NameQ29983
Description of TargetMHC class I polypeptide-related sequence A
Uniprot IDhttps://www.uniprot.org/uniprot/Q29983
Protein Accession #NP_000238.1
Nucleotide Accession #NM_000247.2
Protein Size (# AA)383
Molecular Weight43 kDa
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