MICA Antibody (OAPB00542)

Catalog No: OAPB00542

Size:100UG

Western blot analysis of MICA in A-20 cell lysate with MICA antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.

Immunohistochemistry of MICA in human colon tissue with MICA antibody at 10 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid. MICA Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: MICA antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MICA. The immunogen is located within the last 50 amino acids of MICA.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-MICA antibody is Catalog # OEPB02659
• Application Info: MICA antibody can be used for detection of MICA by Western blot at 0.5 - 1 ug/ml. (Optimal dilution should be determined by user)
• Reference: Rudolph MG, Stanfield RL and Wilson IA. How TCRs bind MHCs, peptides, and coreceptors. Annu. Rev. Immunol.2006; 24:419-66.;Bahram S, Bresnahan M, Geraghty DE, et al. A second lineage of mammalian major histocompatibility complex I genes. Proc. Natl. Acad. Sci. USA1994; 91:6259-63.;Bauer S, Groh V, Wu J, et al. Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science1999; 285:727-9.;Maccalli C, Pende D, Castelli C, et al. NKG2D engagement of colorectal cancer-specific T cells strengthens TCR-mediated antigen stimulation and elicits TCR independent anti-tumor activity. Eur. J. Immunol.2003; 33:2033-43.

Target Info

• Gene Symbol: MICA
• Gene Full Name: MHC class I polypeptide-related sequence A
• Alias Symbols: HLA class I antigen;major histocompatibility complex class I chain-related protein A;MHC class I chain-related protein A;MHC class I polypeptide-related sequence A;MHC class I related chain A;MHC class I related sequence A;MIC-A;PERB11.1;stress inducible class I homolog;truncated MHC class I polypeptide-related sequence A;truncated MICA.
• NCBI Gene Id: 100507436|4276
• Protein Name: MHC class I polypeptide-related sequence A|MHC class I polypeptide-related sequence A isoform 1 (MICA*001) precursor [Homo sapiens]
• Description of Target: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
• Uniprot ID: Q29983
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_000238
• Molecular Weight: Predicted: 42 kDa Observed: 46 kDa