Catalog No: OKCD01243 (Formerly GWB-KBBUQ5)
Size:96WELLS
Price: $700.00
SKU
OKCD01243
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Datasheets/ManualsPrintable datasheet for MGP ELISA Kit (Rat) (OKCD01243)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)81-95%96-103%78-96%91-104%
EDTA plasma(n=5)88-101%98-105%78-98%84-101%
heparin plasma(n=5)89-96%89-98%85-96%82-93%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Gla Protein (MGP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Gla Protein (MGP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Gla Protein (MGP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Matrix Gla Protein (MGP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)89-9793
EDTA plasma(n=5)84-10398
heparin plasma(n=5)88-9793
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Gla Protein (MGP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Gla Protein (MGP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Mgp Microplate96 Wells (12 x 8 Well Strips)
Mgp Lyophilized Standard2 x 50 ng
100X Biotinylated Mgp Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.28 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Matrix Gla Protein (MGP). No significant cross-reactivity or interference between Matrix Gla Protein (MGP) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMgp
Gene Full Namematrix Gla protein
Alias Symbolsmatrix Gla protein;Mglap.
NCBI Gene Id25333
Protein NameMatrix Gla protein
Description of TargetAssociates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation.
Uniprot IDP08494
Protein Accession #NP_036994
Nucleotide Accession #NM_012862.1
Protein Size (# AA)103
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