Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OCOA20804
Size:20UG
Price: $169.00
SKU
OCOA20804
Availability: Domestic: within 1 week delivery | International: 1 week
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

MGMT Protein Lysate (OCOA20804)

Datasheets/ManualsPrintable datasheet for MGMT Protein Lysate (OCOA20804)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionMGMT HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolMGMT
Gene Full NameO-6-methylguanine-DNA methyltransferase
Alias Symbols6-O-methylguanine-DNA methyltransferase;methylated-DNA--protein-cysteine methyltransferase;methylguanine-DNA methyltransferase;O6-methylguanine-DNA methyltransferase;O-6-methylguanine-DNA-alkyltransferase.
NCBI Gene Id4255
Protein NameB4DEE8, NM_002412, NP_002403, P16455
Description of TargetcDNA FLJ56323, highly similar to Methylated-DNA--protein-cysteinemethyltransferase (EC 2.1.1.63), Homo sapiens O-6-methylguanine-DNA methyltransferase (MGMT), mRNA, Methylated-DNA--protein-cysteine methyltransferase, methylated-DNA--protein-cysteine methyltransferase [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P16455
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_002403
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_002412
Molecular Weight24.9 kDa
Write Your Own Review
You're reviewing:MGMT Protein Lysate (OCOA20804)
Your Rating