MGMT ELISA Kit (Rat) (OKEH07066)

Catalog No: OKEH07066

Size:96T

Rat MGMT ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Mgmt ELISA Kit (Rat) (OKEH07066) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mgmt has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mgmt is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mgmt captured in the well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into matrix = 97%
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
  Intra-Assay CV <4.5%
  Inter-Assay CV <8.8%
• ELISA Kit Component:
  • Mgmt Microplate: 96 Wells (12 x 8 Well strips)
  • Mgmt Lyophilized Standard: 2 x 10 ng
  • 100X Mgmt HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.081 ng/mL
• Specificity: Natural and recombinant Rat Methylated-DNA--protein-cysteine methyltransferase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Mgmt
• Gene Full Name: O-6-methylguanine-DNA methyltransferase
• Alias Symbols: 0-6-methylguanine-DNA methyltransferase;6-O-methylguanine-DNA methyltransferase;methylated-DNA--protein-cysteine methyltransferase;O(6)-alkylguanine-DNA alkyltransferase;O6-alkylguanine-DNA alkyltransferase;O6-methylguanine-DNA methyltranferase;O-6-methylguanine-DNA-alkyltransferase.
• NCBI Gene Id: 25332
• Protein Name: P24528
• Description of Target: Methylated-DNA--protein-cysteine methyltransferase
• Uniprot ID: https://www.uniprot.org/uniprot/P24528
• Protein Accession #: NP_036993
• Nucleotide Accession #: NM_012861