Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD02640
Size:96WELLS
Price: $725.00
SKU
OKCD02640
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for MGMT ELISA Kit (Rat) (OKCD02640)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain O-6-Methylguanine DNA Methyltransferase (MGMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of O-6-Methylguanine DNA Methyltransferase (MGMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78.12-5000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level O-6-Methylguanine DNA Methyltransferase (MGMT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level O-6-Methylguanine DNA Methyltransferase (MGMT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MGMT Microplate96 Wells (12 x 8 Well strips)
MGMT Lyophilized Standard2
100X Biotinylated MGMT Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity27 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of O-6-Methylguanine DNA Methyltransferase (MGMT). No significant cross-reactivity or interference between O-6-Methylguanine DNA Methyltransferase (MGMT) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolMgmt
Gene Full NameO-6-methylguanine-DNA methyltransferase
Alias Symbols0-6-methylguanine-DNA methyltransferase;6-O-methylguanine-DNA methyltransferase;methylated-DNA--protein-cysteine methyltransferase;O(6)-alkylguanine-DNA alkyltransferase;O6-alkylguanine-DNA alkyltransferase;O6-methylguanine-DNA methyltranferase;O-6-methylguanine-DNA-alkyltransferase.
NCBI Gene Id25332
Protein NameMethylated-DNA--protein-cysteine methyltransferase
Description of TargetInvolved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Uniprot IDP24528
Protein Accession #NP_036993.1
Nucleotide Accession #NM_012861.1
Protein Size (# AA)209
Write Your Own Review
You're reviewing:MGMT ELISA Kit (Rat) (OKCD02640)
Your Rating
We found other products you might like!