Catalog No: OKCD00652 (Formerly GWB-KBBXT8)
Size:96WELLS
Price: $650.00
SKU
OKCD00652
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for MGEA5 ELISA Kit (Human) (OKCD00652) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Meningioma Expressed Antigen 5 (MGEA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Meningioma Expressed Antigen 5 (MGEA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Meningioma Expressed Antigen 5 (MGEA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Meningioma Expressed Antigen 5 (MGEA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 1.56-100ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Meningioma Expressed Antigen 5 (MGEA5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Meningioma Expressed Antigen 5 (MGEA5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Urine | ||||||||||||||||||||||
Sensitivity | < 0.55 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of N-Acetyl Beta-D-Glucosaminidase (NAGase). No significant cross-reactivity or interference between N-Acetyl Beta-D-Glucosaminidase (NAGase) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | OGA |
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Gene Full Name | O-GlcNAcase |
Alias Symbols | beta-hexosaminidase;beta-N-acetylglucosaminidase;beta-N-acetylhexosaminidase;bifunctional protein NCOAT;hyaluronidase in meningioma;MEA5;meningioma expressed antigen 5 (hyaluronidase);meningioma-expressed antigen 5;MGEA5;N-acetyl-beta-D-glucosaminidase;N-acetyl-beta-glucosaminidase;NCOAT;nuclear cytoplasmic O-GlcNAcase and acetyltransferase;protein O-GlcNAcase. |
NCBI Gene Id | 10724 |
Protein Name | Protein O-GlcNAcase |
Description of Target | Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714). |
Uniprot ID | O60502 |
Protein Accession # | NP_001135906 |
Nucleotide Accession # | NM_001142434.1 |
Protein Size (# AA) | 916 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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