MGAT3 Antibody (OAGA00949)

Catalog No: OAGA00949

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
OAGA00949 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MGAT3(OAGA00949) antibody at 1:500 dilution.

Immunofluorescence analysis of methanol-fixed HeLa, using GnT-III(OAGA00949) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]
  Positive Controls: HeLa, HepG2
  Cellular Localization: Golgi apparatus membrane; Single-pass type II membrane protein
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 260 and 525 of MGAT3 (Uniprot ID#Q09327)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.96 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: MGAT3
• Gene Full Name: Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
• Alias Symbols: GNT3, GNT-III
• NCBI Gene Id: 4248
• Protein Name: Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
• Uniprot ID: Q09327
• Protein Accession #: NP_001091740.1
• Molecular Weight: 61 kDa