Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH08248
Size:96T
Price: $725.00
SKU
OKEH08248
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology MFN2 ELISA Kit (Human) (OKEH08248) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MFN2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MFN2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MFN2 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 83% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=5.7% (n = 20)
Mean Inter-assay CV%: <=7.0% (n = 20)
ELISA Kit Component
ComponentAmount
MFN2 Microplate96 Wells (12 x 8 Well strips)
MFN2 Lyophilized Standard2
100X Biotinylated MFN2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:11181170, PubMed:11950885, PubMed:28114303). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:28114303). Overexpression induces the formation of mitochondrial networks (PubMed:28114303). Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (Probable). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (By similarity). Plays an important role in the regulation of vascular smooth muscle cell proliferation (By similarity). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) (PubMed:23620051). Is required for PARK2 recruitment to dysfunctional mitochondria (PubMed:23620051). Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (By similarity). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
::Subcellular Location: Mitochondrion outer membrane Multi-pass membrane protein Colocalizes with BAX during apoptosis.
::Unigene: Hs.376681
SMR: O95140
String: 9606.ENSP00000235329
::KEGG: hsa:9927
MIM: 601152
Pfam: PF00350
Reconstitution and StorageStore as indicated in product manual.
Sensitivity35pg/mL
SpecificityNatural and recombinant Human Mitofusin-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMFN2
Gene Full Namemitofusin 2
Alias SymbolsCMT2A;CMT2A2;CMT2A2A;CMT2A2B;CPRP1;HMSN6A;HSG;hyperplasia suppressor;MARF;mitochondrial assembly regulatory factor;mitofusin-2;transmembrane GTPase MFN2.
NCBI Gene Id9927
Protein NameO95140
Description of TargetMitofusin-2
Uniprot IDhttps://www.uniprot.org/uniprot/O95140
Protein Accession #NP_001121132.1
Nucleotide Accession #NM_001127660.1
Write Your Own Review
You're reviewing:MFN2 ELISA Kit (Human) (OKEH08248)
Your Rating