MFN1 ELISA Kit (Human) (OKEH08227)

Catalog No: OKEH08227

Size:96T

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology MFN1 ELISA Kit (Human) (OKEH08227) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MFN1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MFN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MFN1 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.7% (n = 20)
  Mean Inter-assay CV%: <=7.5% (n = 20)
• ELISA Kit Component:
  • MFN1 Microplate: 96 Wells (12 x 8 Well strips)
  • MFN1 Lyophilized Standard: 2
  • 100X Biotinylated MFN1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12475957, PubMed:12759376, PubMed:27920125, PubMed:28114303). Membrane clustering requires GTPase activity (PubMed:27920125). It may involve a major rearrangement of the coiled coil domains (PubMed:27920125, PubMed:28114303). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:12475957, PubMed:12759376). Overexpression induces the formation of mitochondrial networks (in vitro) (PubMed:12759376). Has low GTPase activity (PubMed:27920125, PubMed:28114303).
  Subcellular Location: Isoform 2 Cytoplasm
  Unigene: Hs.478383
  SMR: Q8IWA4
  String: 9606.ENSP00000263969
  KEGG: hsa:55669
  MIM: 608506
  Pfam: PF00350
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Human Mitofusin-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MFN1
• Gene Full Name: mitofusin 1
• Alias Symbols: fzo homolog;hfzo1;hfzo2;mitochondrial transmembrane GTPase Fzo-1;mitochondrial transmembrane GTPase FZO-2;mitofusin-1;putative transmembrane GTPase;transmembrane GTPase MFN1.
• NCBI Gene Id: 55669
• Protein Name: Q8IWA4
• Description of Target: Mitofusin-1
• Uniprot ID: https://www.uniprot.org/uniprot/Q8IWA4
• Protein Accession #: NP_284941.2
• Nucleotide Accession #: NM_033540.2