MFN1 Antibody - N-terminal region (OABB01708)

Catalog No: OABB01708

Size:100UG

Anti- Mitofusin 1 Picoband antibody, OABB01708, Western blotting
All lanes: Anti Mitofusin 1 (OABB01708) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Mouset Cardiac Muscle Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Predicted bind size: 84 kDa
Observed bind size: 84 kDa

Anti- Mitofusin 1 Picoband antibody, OABB01708, Western blotting
All lanes: Anti Mitofusin 1 (OABB01708) at 0.5ug/ml
WB: Recombinant Human Mitofusin 1 Protein 0.5ng
Predicted bind size: 45 kDa
Observed bind size: 45 kDa

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Western blot
• Additional Information: Notes: WB: The detection limit for Mitofusin 1 is approximately 0.25ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 80­90 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion, and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers, either with itself or MFN­2, and to undergo ubiquitination by MARCH5. MFN­1 has two key domains. One is a coiled­coil region that mediates MFN­1: MFN­1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Mitofusin-1(MFN1) detection. Tested with WB in Human;Mouse;Rat.

Target Info

• Gene Symbol: MFN1
• Gene Full Name: mitofusin 1
• Alias Symbols: fzo homolog;hfzo1;hfzo2;mitochondrial transmembrane GTPase Fzo-1;mitochondrial transmembrane GTPase FZO-2;mitofusin-1;putative transmembrane GTPase;transmembrane GTPase MFN1.
• NCBI Gene Id: 55669
• Protein Name: Mitofusin-1
• Description of Target: Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12475957, PubMed:12759376, PubMed:27920125, PubMed:28114303). Membrane clustering requires GTPase activity (PubMed:27920125). It may involve a major rearrangement of the coiled coil domains (PubMed:27920125, PubMed:28114303). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:12475957, PubMed:12759376). Overexpression induces the formation of mitochondrial networks (in vitro) (PubMed:12759376). Has low GTPase activity (PubMed:27920125, PubMed:28114303).
• Uniprot ID: Q8IWA4
• Molecular Weight: 84100 MW