METRN ELISA Kit (Mouse) (OKCD02724)

Catalog No: OKCD02724

Size:96WELLS

Mouse METRN ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	93-101%	93-102%	88-104%	78-92%
  EDTA Plasma (n=5)	81-96%	78-90%	87-101%	83-102%
  Heparin Plasma (n=5)	88-102%	93-101%	98-105%	95-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Meteorin (METRN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Meteorin (METRN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Meteorin (METRN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Meteorin (METRN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86-93	89
  EDTA Plasma (n=5)	96-104	101
  Heparin Plasma(n=5)	84-105	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Meteorin (METRN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Meteorin (METRN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-METRN Microplate: 96 Wells (12 x 8 Well strips)
  • METRN Lyophilized Standard: 2
  • 100X Biotinylated METRN Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 5.6 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Meteorin (METRN). No significant cross-reactivity or interference between Meteorin (METRN) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Metrn
• Gene Full Name: meteorin, glial cell differentiation regulator
• Alias Symbols: 1810034B16Rik;EyeLinc16;Hypoxia/reoxygenation regulatory factor;hypoxia/reoxygenation regulatory factor in astrocytes;Hyrac;meteorin.
• NCBI Gene Id: 70083
• Protein Name: Meteorin
• Description of Target: Involved in both glial cell differentiation and axonal network formation during neurogenesis. Promotes astrocyte differentiation and transforms cerebellar astrocytes into radial glia. Also induces axonal extension in small and intermediate neurons of sensory ganglia by activating nearby satellite glia.
• Uniprot ID: Q8C1Q4
• Protein Accession #: NP_598480.1
• Nucleotide Accession #: NM_133719.2
• Protein Size (# AA): 291