Methylmalonic Acid ELISA Kit (OKEH02609)

Catalog No: OKEH02609 (Formerly GWB-2KCO48)

Size:96T

Methylmalonic Acid ELISA Kit (OKEH02609) standard curve.

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Methylmalonic Acid ELISA Kit (OKEH02609) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Methylmalonic Acid antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Methylmalonic Acid and incubated. The Methylmalonic Acid found in the sample or standards competes with the biotinylated Methylmalonic Acid for limited binding sites on the immobilized anti-Methylmalonic Acid antibody. Excess unbound biotinylated Methylmalonic Acid and sample or standard Methylmalonic Acid is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Methylmalonic Acid captured in the well and inversely proportional to the amount of Methylmalonic Acid which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 110%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.1% (n = 20)
  Mean Inter-assay CV%: < 10.2% (n = 20)
• ELISA Kit Component:
  • Anti-Methylmalonic Acid Microplate: 96 Wells (12 x 8 Well strips)
  • Methylmalonic Acid Lyophilized Standard: 2 x 20 ng
  • Sample Diluent: 1 x 20 mL
  • 100X Methylmalonic Acid-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: MMA
  Target Name: Methylmalonic Acid
  Pubchem: 487
  Chemical formula: C4H6O4
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 0.099 ng/mL
• Specificity: Natural and recombinant General Methylmalonic Acid
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: MMA, Methylmalonic Acid, MMA
• Protein Name: Methylmalonic Acid
• Description of Target: Methylmalonic acid (MMA) is a dicarboxylic acid that is a C-methylated derivative of malonate. The coenzyme A linked form of methylmalonic acid, methylmalonyl-CoA, is converted into succinyl-CoA by methylmalonyl-CoA mutase, in a reaction that requires vitamin B12 as a cofactor. In this way, it enters the Krebs cycle, and is thus part of one of the anaplerotic reactions.