METAP2 ELISA Kit (Mouse) (OKEH03636)

Catalog No: OKEH03636

Size:96T

Mouse METAP2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology METAP2 ELISA Kit (Mouse) (OKEH03636) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Metap2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Metap2 and incubated. The Metap2 found in the sample or standards competes with the biotinylated Metap2 for limited binding sites on the immobilized anti-human Metap2 antibody. Excess unbound biotinylated Metap2 and sample or standard Metap2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Metap2 captured in the well and inversely proportional to the amount of Metap2 which was contained in the sample or standard.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 4.3% (n = 20)
  Mean Inter-assay CV%: <= 6.5% (n = 20)
• ELISA Kit Component:
  • Anti-Metap2 Microplate: 96 Wells (12 x 8 Well strips)
  • Metap2 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Metap2-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.39 ng/mL
• Specificity: Natural and recombinant Mouse Methionine aminopeptidase 2
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Metap2
• Gene Full Name: methionine aminopeptidase 2
• Alias Symbols: 4930584B20Rik;A930035J23Rik;AI047573;AL024412;Amp2;AU014659;eIF-2-associated p67;initiation factor 2-associated 67 kDa glycoprotein;initiation factor 2-associated protein (p67);MAP 2;metAP 2;methionine aminopeptidase 2;Mnpep;p6;p67;p67eIF2;Peptidase M;peptidase M 2.
• NCBI Gene Id: 56307
• Protein Name: O08663
• Description of Target: Methionine aminopeptidase 2
• Uniprot ID: https://www.uniprot.org/uniprot/O08663
• Protein Accession #: NP_062622.1
• Nucleotide Accession #: NM_019648.3
• Protein Size (# AA): 478
• Molecular Weight: 53 kDa