METAP2 ELISA Kit (Human) (OKCD02723)

Catalog No: OKCD02723

Size:96WELLS

Human METAP2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methionyl Aminopeptidase 2 (METAP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methionyl Aminopeptidase 2 (METAP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methionyl Aminopeptidase 2 (METAP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Methionyl Aminopeptidase 2 (METAP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methionyl Aminopeptidase 2 (METAP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methionyl Aminopeptidase 2 (METAP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-METAP2 Microplate: 96 Wells (12 x 8 Well strips)
  • METAP2 Lyophilized Standard: 2
  • 100X Biotinylated METAP2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Methionyl Aminopeptidase 2 (METAP2). No significant cross-reactivity or interference between Methionyl Aminopeptidase 2 (METAP2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: METAP2
• Gene Full Name: methionyl aminopeptidase 2
• Alias Symbols: eIF-2-associated p67 homolog;initiation factor 2-associated 67 kDa glycoprotein;MAP2;methionine aminopeptidase 2;MNPEP;p67eIF2;Peptidase M;peptidase M 2;testicular tissue protein Li 17.
• NCBI Gene Id: 10988
• Protein Name: Methionine aminopeptidase 2
• Description of Target: Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
• Uniprot ID: P50579
• Protein Accession #: NP_001304111.1
• Nucleotide Accession #: NM_001317182.1
• Protein Size (# AA): 478