Metanephrine ELISA kit (Human) (OKWB10529)

Catalog No: OKWB10529

Size:96T

Human Metanephrine ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva Systems Biology Metanephrine ELISA Kit (Human) (OKEH00637) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Metanephrine antibody and blocked. Sample or standards are added to the wells along with a fixed quantity of biotinylated Metanephrine and incubated. The Metanephrine found in the sample or standards competes with the biotinylated Metanephrine for limited binding sites on the immobilized anti-Metanephrine antibody. Excess unbound biotinylated Metanephrine and sample or standard Metanephrine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Metanephrine captured in the well and inversely proportional to the amount of Metanephrine which was contained in the sample or standard.
• ELISA Kit Range: 15.625-1000pg/ml
• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Metanephrine was tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Serping1 was tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10%
  Inter-Assay CV: <12%
• ELISA Kit Component:
  • Metanephrine Sulfate Microplate: 96 Wells (12 x 8 Well Strips)
  • Metanephrine Lyophilized Standard: 2 x 20 ng
  • 100X Metanephrine Biotin Complex: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Additional Information: CAS: 5001-33-2
  PubChem: 21100
  Molecular Formula: C10H15NO3
  
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.094 ng/mL
• Assay Info: Assay Methodology: Quantitative Competitive Inhibition ELISA

Target Info

• Alias Symbols: 4-(1-hydroxy-2-methylamino-ethyl)-2-methoxy-phenol, Metanephrine, Metadrenaline, (+/-)-Metanephrine, 3-Methoxyadrenaline, M-O-Methyladrenaline, 5001-33-2
• Protein Name: Metanephrine
• Description of Target: Metanephrine is a metabolite of epinephrine created by action of catechol O-methyltransferase on epinephrine. Technically it is a product of epinephrine O-methylation. It is a commonly occurring, pharmacologically and physiologically inactive metabolite of epinephrine. The measurement of plasma free metanephrines is considered to be the best tool in the diagnosis of pheochromocytoma, a rare kind of adrenal medullary neoplasm. In adrenal chromaffin cells, leakage of norepinephrine and epinephrine from storage granules leads to substantial intracellular production of the O-methylated metabolite metanephrine. In fact, the adrenals constitute the single largest source out of any organ system including the liver for circulating metanephrine. In humans, about 93 percent of circulating metanephrine is derived from catecholamines metabolized within adrenal chromaffin cells.
• Protein Size (# AA): 197.231 g/mol