MESP1 Antibody - N-terminal region (ARP39374_P050)

Catalog#: ARP39374_P050

• Size: 100 ul

• Gene Symbol: MESP1
• Official Gene Full Name: Mesoderm posterior 1 homolog (mouse)
• NCBI Gene Id: 55897
• Protein Name: Mesoderm posterior protein 1
• Swissprot Id: Q9BRJ9
• Protein Accession #: NP_061140
• Nucleotide Accession #: NM_018670
• Alias Symbols: MGC10676, bHLHc5
• Replacement Item: This antibody may replace item sc-130461 from Santa Cruz Biotechnology.
• Description of Target: MESP1 is a transcription factor. MESP1 plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. MESP1 defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways.
• Protein Size (# AA): 268
• Molecular Weight: 28kDa
• Host: Rabbit
• Clonality: Polyclonal
• Purification: Affinity Purified
• Application: WB
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express MESP1.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MESP1
• Predicted Species Reactivity: Dog, Human
• Tested Species Reactivity: Human
• Predicted Homology Based on Immunogen Sequence: Dog: 85%; Human: 100%
• Peptide Sequence: Synthetic peptide located within the following region: SLVSSPDSWGSTPADSPVASPARPGTLRDPRAPSVGRRGARSSRLGSGQR
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Blocking Peptide: For anti-MESP1 (ARP39374_P050) antibody is Catalog # AAP39374 (Previous Catalog # AAPP21385)
• Target Reference: David,R., (2008) Nat. Cell Biol. 10 (3), 338-345
• Publications:

  Cao, N. et al. Highly efficient induction and long-term maintenance of multipotent cardiovascular progenitors from human pluripotent stem cells under defined conditions. Cell Res. 23, 1119-32 (2013). WB, Dog, Human 23896987

  Hu, J; Wang, Y; Jiao, J; Liu, Z; Zhao, C; Zhou, Z; Zhang, Z; Forde, K; Wang, L; Wang, J; Baylink, DJ; Zhang, XB; Gao, S; Yang, B; Chen, YE; Ma, PX; Patient-specific cardiovascular progenitor cells derived from integration-free induced pluripotent stem cells for vascular tissue regeneration. 73, 51-9 (2015). WB, Dog, Human 26398309

  Tan, Y; Han, P; Gu, Q; Chen, G; Wang, L; Ma, R; Wu, J; Feng, C; Zhang, Y; Wang, L; Hu, B; Li, W; Hao, J; Zhou, Q; Generation of Clinical-grade Functional Cardiomyocytes from Human Embryonic Stem Cells in Chemically Defined Conditions. , (2016). WB, Dog, Human 27943600

  Tennis, M. A. et al. Prostacyclin inhibits non-small cell lung cancer growth by a frizzled 9-dependent pathway that is blocked by secreted frizzled-related protein 1. Neoplasia 12, 244-53 (2010). WB, Dog, Human 20335662

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

• IHC Tips & Tricks
• ICC Tips & Tricks
• ELISA Tips & Tricks
• WB/IB Tips & Tricks
• IP Tips & Tricks

See our General FAQ page.

Product Protocols: MESP1 antibody tested with Human 293T Cells (ARP39374_P050)