MERTK ELISA Kit (Human) (OKCD08626)

Catalog No: OKCD08626

Size:96WELLS

MERTK ELISA Kit (Human) (OKCD08626) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Mer Proto Oncogene Tyrosine Kinase (MERTK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Mer Proto Oncogene Tyrosine Kinase (MERTK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Mer Proto Oncogene Tyrosine Kinase (MERTK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C-Mer Proto Oncogene Tyrosine Kinase (MERTK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C-Mer Proto Oncogene Tyrosine Kinase (MERTK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C-Mer Proto Oncogene Tyrosine Kinase (MERTK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • MERTK Microplate: 96 Wells (12 x 8 Well strips)
  • MERTK Lyophilized Standard: 2
  • 100X MERTK HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.113ng/mL

Target Info

• Gene Symbol: MERTK
• Gene Full Name: MER proto-oncogene, tyrosine kinase
• Alias Symbols: c-Eyk;c-mer;c-mer proto-oncogene tyrosine kinase;MER;MER receptor tyrosine kinase;proto-oncogene c-Mer;receptor tyrosine kinase MerTK;RP38;STK kinase;Tyro12;tyrosine-protein kinase Mer.
• NCBI Gene Id: 10461
• Protein Name: Tyrosine-protein kinase Mer
• Description of Target: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
• Uniprot ID: Q12866
• Protein Accession #: NP_006334.2
• Nucleotide Accession #: NM_006343.2