MER/MERTK ELISA Kit (Mouse) (OKBB00637)

Catalog No: OKBB00637

Size:96 Wells

Mouse MER/MERTK ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse MER ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MER has been precoated onto 96-well plates. Standards(sf21, E23-F498) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MER is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse MER amount of sample captured in plate.
• ELISA Kit Range: 78 pg/ml - 5,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	568	1965	3223	790	2135	3632
  SD	25.56	102.18	183.7	45.82	140.91	257.9
  CV%	4.5	5.2	5.7	5.8	6.6	7.1

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse MER Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse MER Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse MER Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, Cell lysates, tissue homogenates and serum
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse MER
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: sf21; Immunogen sequence: E23-F498
• Description: For quantitative detection of mouse MER in cell culture supernatants, cell lysates and serum.

Target Info

• Gene Symbol: MERTK
• Gene Full Name: c-mer proto-oncogene tyrosine kinase
• Alias Symbols: C mer, c mer proto oncogene tyrosine kinase, MER receptor tyrosine kinase, Mertk, MERTK_HUMAN, Proto-oncogene c-Mer, Receptor tyrosine kinase MerTK, RP38, STK kinase, Tyrosine-protein kinase Mer
• NCBI Gene Id: 17289
• Protein Name: Tyrosine-protein kinase Mer
• Description of Target: Proto-oncogene tyrosine-protein kinase MER, also called MERTK is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. This gene is mapped to 2q13. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. This gene also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
• Uniprot ID: Q60805
• Protein Accession #: NP_032613.1
• Nucleotide Accession #: NM_008587.1