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Catalog No: OKCD00669 (Formerly GWB-KBBZN5)
Size:96WELLS
Price: $700.00
SKU
OKCD00669
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Datasheets/ManualsPrintable datasheet for MEPE ELISA Kit (Rat) (OKCD00669)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)94-101%98-105%97-105%96-105%
EDTA plasma(n=5)86-94%80-97%85-104%97-105%
heparin plasma(n=5)96-104%97-105%82-95%79-89%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Extracellular Phosphoglycoprotein (MEPE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Extracellular Phosphoglycoprotein (MEPE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Extracellular Phosphoglycoprotein (MEPE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Matrix Extracellular Phosphoglycoprotein (MEPE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)94-10298
EDTA plasma(n=5)89-9792
heparin plasma(n=5)91-10295
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Extracellular Phosphoglycoprotein (MEPE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Extracellular Phosphoglycoprotein (MEPE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Mepe Microplate96 Wells (12 x 8 Well Strips)
Mepe Lyophilized Standard2 x 100 ng
100X Biotinylated Mepe Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 0.62 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Matrix Extracellular Phosphoglycoprotein (MEPE). No significant cross-reactivity or interference between Matrix Extracellular Phosphoglycoprotein (MEPE) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMepe
Gene Full Namematrix extracellular phosphoglycoprotein
Alias Symbolsmatrix extracellular phosphoglycoprotein;matrix extracellular phosphoglycoprotein with ASARM motif (bone);OF45;osteoblast/osteocyte factor 45;osteoregulin.
NCBI Gene Id79110
Protein NameMatrix extracellular phosphoglycoprotein
Description of TargetRegulates renal phosphate excretion (By similarity). Regulates bone mineralization by osteoblasts and cartilage mineralization by chondrocytes (By similarity). Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage (By similarity). Increases dental pulp stem cell proliferation.
Uniprot IDD6C6P2
Protein Accession #NP_077056
Nucleotide Accession #NM_024142.1
Protein Size (# AA)435
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