MEP1B ELISA Kit (Human) (OKEH04270)

Catalog No: OKEH04270

Size:96T

Human MEP1B ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology MEP1B ELISA Kit (Human) (OKEH04270) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MEP1B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MEP1B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MEP1B captured in the well.
• ELISA Kit Range: 1.56-100ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 5.7% (n = 20)
  Mean Inter-assay CV%: <= 8.9% (n = 20)
• ELISA Kit Component:
  • MEP1B Microplate: 96 Wells (12 x 8 Well Strips)
  • MEP1B Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • MEP1B Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.94 ng/mL
• Specificity: Natural and recombinant Human Meprin A subunit beta
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MEP1B
• Gene Full Name: meprin A subunit beta
• Alias Symbols: endopeptidase-2;meprin A subunit beta;meprin A, beta;meprin B;N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit;N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta;PABA peptide hydrolase;PPH beta.
• NCBI Gene Id: 4225
• Protein Name: Q16820
• Description of Target: Meprin A subunit beta
• Uniprot ID: https://www.uniprot.org/uniprot/Q16820
• Protein Accession #: NP_001295100.1
• Nucleotide Accession #: NM_001308171.1
• Protein Size (# AA): 701
• Molecular Weight: 80 kDa