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Catalog No: OKCD06099
Size:96WELLS
Price: $600.00
SKU
OKCD06099
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Datasheets/ManualsPrintable datasheet for MEP1B ELISA Kit (Human) (OKCD06099)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Meprin A Beta (MEP1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Meprin A Beta (MEP1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Meprin A Beta (MEP1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Meprin A Beta (MEP1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Meprin A Beta (MEP1b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Meprin A Beta (MEP1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
MEP1B Microplate96 Wells (12 x 8 Well strips)
MEP1B Lyophilized Standard2
100X MEP1B HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.117ng/mL
Gene SymbolMEP1B
Gene Full Namemeprin A subunit beta
Alias Symbolsendopeptidase-2;meprin A subunit beta;meprin A, beta;meprin B;N-benzoyl-L-tyrosyl-p-amino-benzoic acid hydrolase beta subunit;N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta;PABA peptide hydrolase;PPH beta.
NCBI Gene Id4225
Protein NameMeprin A subunit beta
Description of TargetMembrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components.
Uniprot IDQ16820
Protein Accession #NP_001295100.1
Nucleotide Accession #NM_001308171.1
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