MELTF ELISA Kit (Human) (OKCD01597)

Catalog No: OKCD01597 (Formerly GWB-KBBRM2)

Size:96WELLS

Human MELTF ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	85-101%	94-101%	81-98%	83-92%
  EDTA Plasma (n=5)	87-95%	82-89%	85-103%	96-104%
  Heparin Plasma (n=5)	80-91%	83-94%	95-105%	94-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanotransferrin (MFI2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanotransferrin (MFI2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanotransferrin (MFI2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Melanotransferrin (MFI2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	89-103	95
  EDTA Plasma (n=5)	92-101	96
  Heparin Plasma (n=5)	98-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanotransferrin (MFI2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanotransferrin (MFI2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MELTF Microplate: 96 Wells (12 x 8 Well Strips)
  • MELTF Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated MELTF Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 12.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Melanotransferrin (MFI2). No significant cross-reactivity or interference between Melanotransferrin (MFI2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MELTF
• Gene Full Name: melanotransferrin
• Alias Symbols: antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5;CD228;MAP97;Melanoma-associated antigen p97;melanotransferrin;membrane-bound transferrin-like protein;MFI2;MTf;MTF1.
• NCBI Gene Id: 4241
• Protein Name: Melanotransferrin
• Description of Target: Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.
• Uniprot ID: P08582
• Protein Accession #: NP_005920.2
• Nucleotide Accession #: NM_005929.5
• Protein Size (# AA): 738